Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

Mesoderm

MeSH Description

It lies laterally to intermediate mesoderm.

Sample

source_name

embryo

tissue

mesoderm

genotype

WT

chip antibody

H3K27me3(Diagenode,Cat.C15410069)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

37939939

Reads aligned (%)

26.3

Duplicates removed (%)

25.0

Number of peaks

140 (qval < 1E-05)

mm9

Number of total reads

37939939

Reads aligned (%)

45.5

Duplicates removed (%)

26.1

Number of peaks

361 (qval < 1E-05)