Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Epiblast
NA
NA

Attributes by original data submitter

Sample

source_name
embryo
tissue
epiblast
genotype
WT
chip antibody
H3K27me3(Diagenode,Cat.C15410069)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
49554709
Reads aligned (%)
36.6
Duplicates removed (%)
16.9
Number of peaks
236 (qval < 1E-05)

mm9

Number of total reads
49554709
Reads aligned (%)
36.4
Duplicates removed (%)
16.8
Number of peaks
223 (qval < 1E-05)

Base call quality data from DBCLS SRA