Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Epiblast
NA
NA

Attributes by original data submitter

Sample

source_name
embryo
tissue
epiblast
genotype
WT
chip antibody
H3K27me3(Diagenode,Cat.C15410069)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
34341495
Reads aligned (%)
38.3
Duplicates removed (%)
24.6
Number of peaks
228 (qval < 1E-05)

mm9

Number of total reads
34341495
Reads aligned (%)
57.2
Duplicates removed (%)
27.4
Number of peaks
510 (qval < 1E-05)

Base call quality data from DBCLS SRA