Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Endoderm

MeSH Description

The inner of the three germ layers of an embryo.

Sample

source_name

embryo

tissue

endoderm

genotype

WT

chip antibody

H3K4me3(home made)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

29744249

Reads aligned (%)

36.5

Duplicates removed (%)

39.1

Number of peaks

14767 (qval < 1E-05)

mm9

Number of total reads

29744249

Reads aligned (%)

61.6

Duplicates removed (%)

43.1

Number of peaks

24007 (qval < 1E-05)