Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Primitive Streak

MeSH Description

A linear band of rapidly proliferating cells that begins near the posterior end of an embryo and grows cranially. Primitive streak is formed during GASTRULATION by the convergent migration of primary ectodermal cells (EPIBLAST). The knot at the tip of the streak is called HENSEN NODE.

Sample

source_name

embryo

tissue

primitive streak

genotype

WT

chip antibody

H3K4me3(home made)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

2139599

Reads aligned (%)

73.2

Duplicates removed (%)

10.3

Number of peaks

802 (qval < 1E-05)

mm9

Number of total reads

2139599

Reads aligned (%)

73.0

Duplicates removed (%)

10.1

Number of peaks

789 (qval < 1E-05)