Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Embryo
Cell type
Embryonic limb
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic limb bud
tissue
Embryonic limb bud
genotype
WT
embryonic stage
E12.5
strain
G4 ESC

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
50.000 cells were used for each reaction of tagmentation and protocol was performed as previously described (Buenrostro JD et al., 2015). Libraries were prepared using the Nextera adaptors and sequenced

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
71373275
Reads aligned (%)
84.8
Duplicates removed (%)
19.7
Number of peaks
23463 (qval < 1E-05)

mm9

Number of total reads
71373275
Reads aligned (%)
84.7
Duplicates removed (%)
19.7
Number of peaks
23404 (qval < 1E-05)

Base call quality data from DBCLS SRA