Antigen

Antigen Class

Histone

Antigen

H3S10ph

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Bone Marrow Derived Macrophage - stimulated/LPS treated

tissue

Bone Marrow Derived Macrophage

stimulation time

60

chip antibody

H3S28ph

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNAseq: RNA was isolated using RNAeasy Kit (Qiagen). Libraries were prepared using Illumina TruSeq protocol. ChIP-seq:As previously described in Josefowicz et al., 2016

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

78913669

Reads aligned (%)

79.5

Duplicates removed (%)

8.8

Number of peaks

581 (qval < 1E-05)

mm9

Number of total reads

78913669

Reads aligned (%)

79.3

Duplicates removed (%)

8.7

Number of peaks

728 (qval < 1E-05)