ChIP was conducted according to protocols adapted from Cecchini et al., 2014. Briefly, asynchronously cycling cells were fixed in either 1% formaldehyde in 1X PBS or in 2mM ethylene glycol bis(succinimidyl succinate (EGS) in 1X PBS followed by 1% formaldehyde. Both fixing reactions were neutralized with 0.125M glycine. Cross-linked chromatin was sonicated so most chromatin was ≤400 bp. Sheared chromatin was then normalized between experimental groups and pre-cleared with protein G Dynabeads and IgG. Pre-cleared chromatin was then incubated with protein G Dynabeads and ChIP antibodies to immunoprecipitate proteins. Libraries were constructed according to the NEBNext Ultra II DNA library prep kit.