Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

embryonic stem (ES) cells

cell type

embryonic stem (ES) cells

genetic background

hybrid 129-C57BL/6

genotype

Adnp KO

antibody

anti H3K27ac (Abcam, ab2749)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 5% of chromatin was set aside as Input control. The chromatin was incubated with antibody and protein A/G coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Kaaij et al, 2019. Libraries were prepared using the NEB Next Ultra II DNA library prep kit according to manufacturers instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

76697792

Reads aligned (%)

95.7

Duplicates removed (%)

9.1

Number of peaks

21975 (qval < 1E-05)

mm9

Number of total reads

76697792

Reads aligned (%)

95.5

Duplicates removed (%)

9.1

Number of peaks

22032 (qval < 1E-05)