GSM3563960: ChIPseq for Rad21 in wt mES cells, replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Rad21
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
embryonic stem (ES) cells
cell type
embryonic stem (ES) cells
genetic background
hybrid 129-C57BL/6
genotype
wt
antibody
anti Rad21 (Abcam, ab992)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 5% of chromatin was set aside as Input control. The chromatin was incubated with antibody and protein A/G coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Kaaij et al, 2019. Libraries were prepared using the NEB Next Ultra II DNA library prep kit according to manufacturers instructions.