Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cell

cell line

E14Tg2a.IV

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIPseq, Lysates were clarified from sonicated cells and transcription factor-DNA complexes were isolated with antibody. For RNAseq, PrepEase RNA Spin Kit (Affymetrix) were used for RNA extraction.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

122801441

Reads aligned (%)

96.0

Duplicates removed (%)

13.4

Number of peaks

518 (qval < 1E-05)

mm9

Number of total reads

122801441

Reads aligned (%)

95.8

Duplicates removed (%)

14.0

Number of peaks

534 (qval < 1E-05)