Antigen

Antigen Class

TFs and others

Antigen

SMARCA4

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived neural cells

NA

NA

Sample

source_name

Neural stem cells

cell type

CD34+ HSPC-derived iPSC-differentiated NSC

passage

12-15

chip antibody

SMARCA4 ChIP grade (abcam 110641)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was done as described previously (Ding et al., 2015) using 20 μl of ChIP-grade SMARCA4 (abcam 110641). DNA was fragmented to 200-500bp with a Covaris water-bath sonicator before the IP. DNA was purified and eluted using a PCR purification kit (Qiagen) in 30 μl HPLC-grade water. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

39121479

Reads aligned (%)

97.3

Duplicates removed (%)

9.5

Number of peaks

56777 (qval < 1E-05)

hg19

Number of total reads

39121479

Reads aligned (%)

97.2

Duplicates removed (%)

9.6

Number of peaks

56725 (qval < 1E-05)