GSM3560272: ATAC-seq Adherent rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived hematopoietic cells
NA
NA
Attributes by original data submitter
Sample
source_name
AML_4_10Adherent_1
cell type
iPSC-derived hematopoietic cells
time-point
day 16 of differentiation
harvest
manual separation
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Samples were processed as follows: nuclei were isolated by washing with ATAC buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2) and lysing with 50 ul of ATAC lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40). 1ml of ATAC buffer was added to dilute lysis buffer Illumina Nextera DNA Sample Preparation Kit