Antigen

Antigen Class

TFs and others

Antigen

ERG

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

human umbilical vein endothelial cells

cell type

Pooled HUVEC

passage

4 to 6

treatment

control

chip antibody

ERG (sc-354X, Santa Cruz Biotechnology)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fixed whole cell lysate was sheared by sonication and subsequently incubated with the ERG antibody to isolate bound DNA fragments. Libraries were prepared with TruSeq ChIP Sample Prep Kit (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

35200477

Reads aligned (%)

98.2

Duplicates removed (%)

73.0

Number of peaks

11246 (qval < 1E-05)

hg19

Number of total reads

35200477

Reads aligned (%)

97.5

Duplicates removed (%)

74.4

Number of peaks

11171 (qval < 1E-05)