Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Bone

Cell type

Sarcoma cell line

NA

NA

Sample

source_name

G1297 cell line

cell line

G1297

treatment

Doxycycline (0.2µg/ml)

antibody

anti-H3K27ac mouse monoclonal antibody, Wako MABI0309

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies; anti-HA mouse monoclonal antibody, Nacalai cat# 06340-54; anti-H3K27ac mouse monoclonal antibody, Wako MABI0309. Libraries were prepared according to the manual of Illumina's Trueseq ChIP sample prep kit set A (Part# IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

26563363

Reads aligned (%)

99.1

Duplicates removed (%)

18.9

Number of peaks

312 (qval < 1E-05)

mm9

Number of total reads

26563363

Reads aligned (%)

98.9

Duplicates removed (%)

18.9

Number of peaks

285 (qval < 1E-05)