Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEF

cell line

MEF

treatment

Doxycycline (0.2µg/ml)

antibody

anti-HA mouse monoclonal antibody, Nacalai cat# 06340-54

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies; anti-HA mouse monoclonal antibody, Nacalai cat# 06340-54; anti-H3K27ac mouse monoclonal antibody, Wako MABI0309. Libraries were prepared according to the manual of Illumina's Trueseq ChIP sample prep kit set A (Part# IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

52987954

Reads aligned (%)

95.5

Duplicates removed (%)

22.3

Number of peaks

34627 (qval < 1E-05)

mm9

Number of total reads

52987954

Reads aligned (%)

95.4

Duplicates removed (%)

22.4

Number of peaks

34606 (qval < 1E-05)