GSM3557716: G1297 Dox ON HA input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Bone
Cell type
Sarcoma cell line
NA
NA
Attributes by original data submitter
Sample
source_name
G1297 cell line
cell line
G1297
treatment
Doxycycline (0.2µg/ml)
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies; anti-HA mouse monoclonal antibody, Nacalai cat# 06340-54; anti-H3K27ac mouse monoclonal antibody, Wako MABI0309. Libraries were prepared according to the manual of Illumina's Trueseq ChIP sample prep kit set A (Part# IP-202-1012).