Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Lung

Cell type

Lung

MeSH Description

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Sample

source_name

Murine cell line

antibody

H3K4me3

strain

C57BL/6

tissue

Lung

cell line

SPC-Cre KP

genotype

KrasG12D p53-/-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde. Pellet was resuspended in complete sonication buffer (20mM Tris pH 8.0, 2mM EDTA, 0.5mM EGTA, 1x protease inhibitors (Roche), 0.5% SDS and 0.5mM PMSF) in 1mL for 1x108 cells DNA libraries were prepared using ThruPlex Kit

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

54657008

Reads aligned (%)

95.1

Duplicates removed (%)

11.2

Number of peaks

29983 (qval < 1E-05)

mm9

Number of total reads

54657008

Reads aligned (%)

95.0

Duplicates removed (%)

11.2

Number of peaks

29952 (qval < 1E-05)