GSM3537287: IL6 KO TFH (1); Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
T cell
strain
C57BL/6
genotype
IL6-/-
tissue
mediastinal Lymph node
cell type
CD4+ T cell
cell subset
TFH
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
OTII (CD45.1+) cells were adoptively transferred into WT (C57Bl/6) and Il6-/- recipient mice. One day later, recipient mice were infected with PR8-OTII. Seven days after infection, 30,000 donor-derived CD45.1+CXCR5hiSLAMloCD4+CD19- TFH and CD45.1+CXCR5loSLAMhi CD4+CD19- Teff cells were sorted from the mLN of WT and Il6-/- recipient mice. Nextera primers were used for library amplification.