OTII (CD45.1+) cells were adoptively transferred into WT (C57Bl/6) and Il6-/- recipient mice. One day later, recipient mice were infected with PR8-OTII. Seven days after infection, 30,000 donor-derived CD45.1+CXCR5hiSLAMloCD4+CD19- TFH and CD45.1+CXCR5loSLAMhi CD4+CD19- Teff cells were sorted from the mLN of WT and Il6-/- recipient mice. Nextera primers were used for library amplification.