GSM3537179: ATAC-Seq: ARID1A-WT1 rep3; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Uterus
Cell type
Endometrial epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
endometrial epithelial cells
cell type
endometrial epithelial cells
genotype
ARID1A WT
biological replicate
1
technical replicate
3
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq library construction was performed as previously described with minor modifications (PMID: 24097267) using 60,000 cells of two independent clones of human endometrial epithelial ARID1AWT and ARID1AKO cells. Transposed DNA was purified using a QIAgen MinElute PCR Purification kit, and was subsequently amplified using NEBNext High Fidelity 2x PCR master mix for 12 cycles. PCR products were purified using Agencourt AMPure XP, and sequencing was performed by JHMI Deep Sequencing and Microarray Core using a NextSeq500 platform with double-end reads of 75 bases.