GSM3529700: 5 029J 009KGenen Pooled Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Myeloid-derived suppressor cells
NA
NA
Attributes by original data submitter
Sample
source_name
MDSC
chip antibody
none
treatment
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. Chromatin was isolated by the addition of lysis buffer, followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp. ChIP reactions were performed in the presence of a constant amount of Drosophila chromatin, corresponding to a minor fraction of the total ChIP DNA, and the Drosophila-specific H2A.v antibody as a means of spike-in normalization amongst different samples. Illumina sequencing libraries were prepared from the ChIP and input DNAs following standard enzymatic steps consisting of end-polishing, dA-addition, and adaptor ligation. The resulting DNA libraries were quantified and sequenced as 150 bp paired-end reads using Illumina's HiSeq 2500. The average size of the sequenced fragments were around 380 bp.