GSM3523540: Rbpj KO Mtg16 IP replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Cbfa2t3
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal crypt
NA
NA
Attributes by original data submitter
Sample
source_name
Intestinal crypt epithelial cells
tissue
Intestinal crypt epithelial cells
genotype/variation
Rbpj KO
chip antibody
Mtg16 (Abcam, ab33072)
timepoint
day4
treatment
Tamoxifen induced RBPJ knock-out
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The samples were washed twice in PBS and resuspended in commercial lysis buffer containing protease inhibitors from the MAGnify Chromatin Immunoprecipitation System kit (Thermo Scientific; 492024). The chromatin was sheared using the Covaris S2 sonicator (Covaris) at 4°C for 10 minutes with the following settings; duty cycle 5%, intensity: 2, cycles per burst: 200, cycle time: 60 seconds. The DNA library for sequencing was prepared using 20 ng of ChIP'ed DNA for the Kapa Hyper Prep kit with 16 cycles of PCR amplification. The quality of the final DNA library was confirmed on the Agilent Tapestation before the samples were submitted to sequencing on the HiSeq 4000 (Illumina).