Rabbit Notch1 mAb (D1E11, Cell Signaling Technology)
cell type
gamma delta T cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cellular lysate was prepared by sonication in detergent-containing buffer after fixation with 0.5% formaldehyde at room temperature for 5min, and then DNA/protein complex was obtained by enzymatic digestion. To repair ChIP-DNA and input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with NEB Next Multiplex Oligos for Illumina (Index Primers Set1).