Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Lymphocytes

MeSH Description

White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.

Sample

source_name

Peripheral blood

cell type

FACS-purified lymphocytes

markers

CRTH2-KLRG1+

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Lymphocyte fractions were obtained from peripheral blood samples (buffy coats) donated by anonymous healthy donors. Cells were FACS-sorted using specific antibodies and YFP as described in detail in the manuscript. Nextera DNA library construction (as described in Buenrostro et al. Nature Methods 2013).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

9058510

Reads aligned (%)

53.3

Duplicates removed (%)

37.7

Number of peaks

11216 (qval < 1E-05)

hg19

Number of total reads

9058510

Reads aligned (%)

53.0

Duplicates removed (%)

37.8

Number of peaks

11174 (qval < 1E-05)