Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

colon

cell line

HCT116

disease

colorectal carcinoma

morphology

epithelial

tissue

colon

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. To generate libraries for high-throughput sequencing, purified DNA fragments were end-repaired, adenylated, ligated to adaptors and PCR amplified for 18 cycles. The PCR products corresponding to 250-450 bps were gel purified, quantified and stored at -80℃ until used for sequencing.For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to illumina NextSeq 500 system for 151 nt pair-end sequencing

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

12141542

Reads aligned (%)

82.4

Duplicates removed (%)

1.9

Number of peaks

368 (qval < 1E-05)

hg19

Number of total reads

12141542

Reads aligned (%)

81.9

Duplicates removed (%)

1.9

Number of peaks

327 (qval < 1E-05)