rabbit anti-GR antibody; Santa Cruz Biotechnolgoy, sc-8992
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitation (IP) was performed on 100μl lysates diluted 1:3 in incubation buffer.Immunoprecipitated material was collected the next day by centrifugation and washed once each with WBI, WBII, LiCl buffer, and twice with EDTA/HEPES buffer (0.5M EDTA and 1M HEPES) using spin columns with 0.35μM pore size filters (MoBiTec).The recovered DNA was purified by the PCR purification kit (Qiagen) according to the manufacturer's protocol, eluting in 50μl of Qiagen Elution buffer RNA libraries were prepared for sequencing using standard Illumina protocols (truseq)