GSM3517477: ESC1 KO KAP1 ChIP; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Trim28
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ES cells (zygotic mutants)
strain
C57BL/6J
tissue
ES cells
genotype
ZFP57 KO (zygotic)
Sex
male
antibody
KAP1 (Abcam, ab10483, lot J0809)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using tale lysis buffer (50mM Tris-Cl pH8.5; 5mM EDTA; 200mM Nacl; 0.25% SDS) and 1 µg/ml Proteinase K for 1 hr. The mixture was purified with phenol/chloroform extraction and precipitated with isopropanol. Paired-end libraries were prepared and sequenced in 150bp reads run according to Illumina instructions.