Cells were trypsinized and single cell suspensions were harvested for STAR Scripts run using C1 Fluidigm Open App chips (10-17um). The open chromatin was transposed by Tn5 transposase. Cell specific barcode was integrated to tagmented DNA of each cell by PCR amplification with indexed primer. The amplified PCR produced was purified by Ampure XP beads and the purified ATACseq library was sent for sequencing.