Antigen

Antigen Class

TFs and others

Antigen

Kdm1a

Cell type

Cell type Class

Neural

Cell type

Medulloblastoma

MeSH Description

A malignant neoplasm that may be classified either as a glioma or as a primitive neuroectodermal tumor of childhood (see NEUROECTODERMAL TUMOR, PRIMITIVE). The tumor occurs most frequently in the first decade of life with the most typical location being the cerebellar vermis. Histologic features include a high degree of cellularity, frequent mitotic figures, and a tendency for the cells to organize into sheets or form rosettes. Medulloblastoma have a high propensity to spread throughout the craniospinal intradural axis. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2060-1)

Sample

source_name

Lsd1 ChIP-seq data derived from murine MG (Myc/Gfi1) medulloblastoma tumor

mouse strain

C57BL/6

tissue type

murine medulloblastoma tumor

chip antibody

Rabbit polyclonal, anti-Lsd1, Abcam #ab17721 (4ug)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

To isolate NSCs, Prominin1+, Lineage-negative (lin-) cells were FACS-sorted from cerebella of 5-7-day-old WT mice. Tumor cells were isolated by enzymatic dissociation of cerebella from adult tumor-bearing mice. All cells were snap-frozen in liquid nitrogen. Frozen tumor tissue was processed for ChIP at ActiveMotif (Carlsbad, CA) according to proprietary protocols. According to Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

29991928

Reads aligned (%)

0.0

Duplicates removed (%)

0.6

Number of peaks

12432 (qval < 1E-05)

mm9

Number of total reads

29991928

Reads aligned (%)

0.0

Duplicates removed (%)

0.6

Number of peaks

12362 (qval < 1E-05)