GSM3510179: input HOM spike-in; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Muscle
Cell type
Muscle satellite cells
NA
NA
Attributes by original data submitter
Sample
source_name
Muscle stem (satellite) cells_Lmna Δ8-11 -/-
strain background
C57BL/6J
genotype/variation
Lmna {delta}8-11 -/-
age
Post natal day 19
cell type
Muscle stem (satellite) cells
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cell extraction protocol: Satellite cells were isolated from hind-limb muscle and fixed before sorting using FACS Cells were sonicated and for spike-in experiments a 5% of sheared drosophila chromatin was added to each sample. Satellite cells from pools of 6-9 mice per genotype were used DNA libraries were prepared for sequencing using standard Illumina protocols