SRX5124734
GSM3508274: Nr4aWT CAR-T cells rep1 tr2 (atac-seq); Mus musculus; ATAC-seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
B16-OVA-hCD19 tumor infiltrating lymphocytes
strain
Nr4a1fl/fl,Nr4a2fl/fl
cell type
CD8+ T cell
treatment
RV_Empty, RV_CAR-T

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Isolated cells were lysed in 10 mM Tris pH 7.5, 10 mM NaCl, 3mM MgCl2, 0.1% NP-40 Lysed cells were treated with Nextera transposase for 30 minutes at 37°C, followed by PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
32092527
Reads aligned (%)
88.7
Duplicates removed (%)
46.3
Number of peaks
19309 (qval < 1E-05)

mm9

Number of total reads
32092527
Reads aligned (%)
88.6
Duplicates removed (%)
46.4
Number of peaks
19279 (qval < 1E-05)

Base call quality data from DBCLS SRA