ChIP-Atlas: SRX5124110

Antigen

Cell type Class: Blood
Cell type: Monocytes-CD14+
Tissue: monocytes
Lineage: mesoderm
Description: Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: 5,000 cells were resuspended in 50 μl of tagmentation mixture (25 μl TD buffer, 2.5 μl of TDE1, 0.5 μl 1% digitonin and 22 μl of water) (Nextera DNA Library Preparation Kit, Illumina) and incubated at 37°C for 30 minutes. After tagmentation, DNA was purified with MinElute PCR Purification kit. Cells were lysed and tagmented following FAST ATACseq protocol (Corces et al. Nat Genet. 2016 October ; 48(10): 1193–1203) and tagmented DNA was amplified as previously described (Buenrostro et al. Nat Methods. 2013; 10:1213–1218). Final libraries were size selected using AMPure XT beads.