Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa cells

chromation digestion

Sonication

treatment

-

cell line

HeLa

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Samples were treated with RNase A and Proteinase K prior to DNA extraction with phenol-chloroform-isoamylalcohol. Libraries were prepared according to NEBNext ChIP-Seq Kit for Illumina sequencing. Adapter were removed by size selection using AMPure XP magnetic beads.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

hg38

Number of total reads

25863512

Reads aligned (%)

98.3

Duplicates removed (%)

4.6

Number of peaks

1094 (qval < 1E-05)

hg19

Number of total reads

25863512

Reads aligned (%)

97.4

Duplicates removed (%)

6.3

Number of peaks

1159 (qval < 1E-05)