Antigen

Antigen Class

TFs and others

Antigen

POU2F1

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

HCT116 cells

tissue

colorectal carcinoma

chip antibody

Oct1 (Pou2f1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody (Oct1: Bethyl A310-610A, H3K4me3: Cell Signaling #9727). Qiagen Qiaquick PCR cleanup kit (Cat # 28104). Elution in nuclease-free water. NEBNext ChIP-Seq Library Prep Master Mix Set (Cat # E6240S) with Unique Dual Index Adapters

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg19

Number of total reads

88529580

Reads aligned (%)

96.3

Duplicates removed (%)

43.3

Number of peaks

9037 (qval < 1E-05)

hg38

Number of total reads

88529580

Reads aligned (%)

96.9

Duplicates removed (%)

42.8

Number of peaks

10497 (qval < 1E-05)