Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mesenchymal stem cell
chip antibody
H3K9me3 (Abcam, catalog# ab8898, lot# GR111948-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody(anti-H3K9me3, abcam, ab8898). Libraries were prepared by library construction NEBNext kit according to the manufacturer’s protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
9597712
Reads aligned (%)
98.0
Duplicates removed (%)
0.2
Number of peaks
549 (qval < 1E-05)

hg19

Number of total reads
9597712
Reads aligned (%)
97.2
Duplicates removed (%)
0.4
Number of peaks
400 (qval < 1E-05)

Base call quality data from DBCLS SRA