Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7

cell line

MCF7

cell type

Breast cancer cell line

sirna

siCtrl

chip-antibody

anti-H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked with 1% Formaldehyde, DNA was sheared using ultrasound and IP was overnight at 4C. ThruPLEX

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

19713143

Reads aligned (%)

99.5

Duplicates removed (%)

12.3

Number of peaks

22722 (qval < 1E-05)

hg19

Number of total reads

19713143

Reads aligned (%)

99.2

Duplicates removed (%)

12.5

Number of peaks

22701 (qval < 1E-05)