Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

T47D

cell line

T47D

cell type

Breast cancer cell line

sirna

siCtrl

chip-antibody

anti-H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked with 1% Formaldehyde, DNA was sheared using ultrasound and IP was overnight at 4C. ThruPLEX

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

26906178

Reads aligned (%)

99.1

Duplicates removed (%)

10.2

Number of peaks

25428 (qval < 1E-05)

hg19

Number of total reads

26906178

Reads aligned (%)

98.7

Duplicates removed (%)

10.8

Number of peaks

25431 (qval < 1E-05)