Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Pro-B cells

NA

NA

Sample

source_name

primary preB cells

strain

C57BL/6

cell type

primary preB cells from bone-marrow

genotype

premalignant

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary pre-B cells were purified from bone marrow of wt, prem and mal mice on the basis of high B220, low IgM and low IgD surface marker expression by flow cytometry. Libraries were prepared using ThruPLEX-FD Prep Kit (Rubicon Genomics Inc.)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

22420870

Reads aligned (%)

97.3

Duplicates removed (%)

5.5

Number of peaks

155 (qval < 1E-05)

mm9

Number of total reads

22420870

Reads aligned (%)

97.2

Duplicates removed (%)

6.1

Number of peaks

153 (qval < 1E-05)