Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Testicular germ cells
NA
NA

Attributes by original data submitter

Sample

source_name
testicular germ cells
diet
control diet
genotype
wild-type
cell type
testicular germ cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell ysates were prepared from sonicated TGCs, and ATF7-DNA complexes were isolated by ATF7 antibody. ChIP-seq libraries were prepared by the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs), and sequenced on Illumina Nextseq (36-bp paired end).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
5806006
Reads aligned (%)
86.0
Duplicates removed (%)
19.9
Number of peaks
75 (qval < 1E-05)

mm9

Number of total reads
5806006
Reads aligned (%)
85.8
Duplicates removed (%)
20.6
Number of peaks
71 (qval < 1E-05)

Base call quality data from DBCLS SRA