Antigen

Antigen Class

TFs and others

Antigen

NCOA2

Cell type

Cell type Class

Uterus

Cell type

Endometrial stromal cells

NA

NA

Sample

source_name

human endometrial stromal cells (hESCs)

cell type

endometrial stromal cells

treatment

10nM beta-estradiol, 100nM medroxyprogesterone acetate, and 1mM cAMP

antibody

SRC-2/NCOA2 (Bethyl A300-346A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody Bethyl A300-346A. ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3'-ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

35761720

Reads aligned (%)

90.5

Duplicates removed (%)

76.0

Number of peaks

543 (qval < 1E-05)

hg19

Number of total reads

35761720

Reads aligned (%)

89.9

Duplicates removed (%)

76.9

Number of peaks

610 (qval < 1E-05)