Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NCOA2

Cell type

Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA

Attributes by original data submitter

Sample

source_name
human endometrial stromal cells (hESCs)
cell type
endometrial stromal cells
treatment
10nM beta-estradiol, 100nM medroxyprogesterone acetate, and 1mM cAMP
antibody
SRC-2/NCOA2 (Bethyl A300-346A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody Bethyl A300-346A. ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3'-ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on HiSeq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
35761720
Reads aligned (%)
20.7
Duplicates removed (%)
76.9
Number of peaks
610 (qval < 1E-05)

hg38

Number of total reads
35761720
Reads aligned (%)
21.8
Duplicates removed (%)
76.0
Number of peaks
543 (qval < 1E-05)

Base call quality data from DBCLS SRA