GSM3498498: TIL Bulk P1 TIM S3; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
TIL_Bulk_P1_TIM
strain background
C57BL/6
cell type
CD8+ T-cells
cell subtype
Slamf6-Tim3+SIINFEKL Tetramer+ CD44+PD-1+CD8+ T cells isolated from B16-ova tumors 22 days post-inoculation after treatment with anti-PD-1 on days 9 and 12
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
CD8+ T cells were isolated from LCMV-infected mice by CD8+ MACS negative selection performed on single-cell suspensions of splenocytes, followed by FACS. CD8+ T cells were isolated from B16-ova tumors by dissecting tumors from the surrounding fascia, mechanically mincing and treating with collagenase P and DNAse I for 10 minutes at 37C. Tumor-infiltrating leukocytes were enriched by CD8+ MACS positive selection (Miltenyi) and FACS isolation. In both contexts, cells were sorted directly into PBS with 2% FBS. Library construction was performed as per standard ATAC-seq protocol, as outlined in Corces et al., Nature Genetics 2016