Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

CD4 T cell

strain/background

C57BL/6

genotype/variation

wild-type

tissue

spleen and lymph nodes

cell type

Tfh cells

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells sorted from activated CD4 T cells, washed with 1X PBS, and nuclei were isolated. The transposition reaction was carried out using Nextera DNA Sample Preparation Kit. Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

30372843

Reads aligned (%)

93.8

Duplicates removed (%)

29.8

Number of peaks

27259 (qval < 1E-05)

mm9

Number of total reads

30372843

Reads aligned (%)

93.6

Duplicates removed (%)

30.0

Number of peaks

27229 (qval < 1E-05)