Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

CD4 T cell

strain/background

C57BL/6

genotype/variation

wild-type

tissue

spleen and lymph nodes

cell type

Activated CD4+ T cells

treatment

IL6

time point

D1

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells sorted from activated CD4 T cells, washed with 1X PBS, and nuclei were isolated. The transposition reaction was carried out using Nextera DNA Sample Preparation Kit. Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

46210759

Reads aligned (%)

93.5

Duplicates removed (%)

39.3

Number of peaks

14528 (qval < 1E-05)

mm9

Number of total reads

46210759

Reads aligned (%)

93.4

Duplicates removed (%)

39.4

Number of peaks

14491 (qval < 1E-05)