Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Lung

Cell type

Lung cancer

NA

NA

Sample

source_name

in vivo

disease state

NSCLC

cell type

lung tumor

genotype

KrasG12D/+;Trp53-/-;GFPi-GFP-2A-rTA;Luc

chip antibody

anti-H3K27ac ab4729 Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Trypsin/Collegenase/Hyarulonydase/DNaseI digestion followed by SDS buffer lysis. ChIP-seq was performed as previously described (Serresi et al., 2016). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

22692515

Reads aligned (%)

89.6

Duplicates removed (%)

66.1

Number of peaks

22033 (qval < 1E-05)

mm9

Number of total reads

22692515

Reads aligned (%)

89.5

Duplicates removed (%)

66.2

Number of peaks

21974 (qval < 1E-05)