Male urogenital sinuses were treated with collagenase to disaggregate into single-cells. For 4C-seq cells were crosslinked with 1% PFA for 10mins.Chromatin was digested with three additions of 400U DpnII, followed by ligation, and a second digest with 400U HindIII. Primers with the barcode for illumina's sequencers were attached to the specific primers for each sample For 4C-seq independent chromosomal libraries from biological replicates were amplified with Tbx18 viewpoint primers. For ATAC-seq, nextera transposon primers were used to amplify sequences.