GSM3495123: ESC BirA Lifminus; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
embryonic stem cells
cell type
J1 embryonic stem (ES) cells
expressing
BirA
culture condition
Lifminus
pulldown reagent
streptavidin-conjugated beads
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After reaching ~80% confluency, ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution (neutralized with 125 mM glycine) J1 mouse ES cells. After sonication and extensive washing, immunoprecipitated DNA was sequenced using Illumina sequencers. After pulldown, DNA was used to generate sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (#E7645S) according to the manufacturer's instructions.