Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

Embryos

NA

NA

Sample

source_name

embryo

tissue

embryo

developmental stage

Early2C

chip-antibody

H3K27me3(Diagenode,Cat.C15410069)

genotype

Setd2-/-

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

27976577

Reads aligned (%)

28.6

Duplicates removed (%)

46.3

Number of peaks

63 (qval < 1E-05)

mm9

Number of total reads

27976577

Reads aligned (%)

28.5

Duplicates removed (%)

46.2

Number of peaks

41 (qval < 1E-05)