Adipose from NICHD Brain and Tissue Bank for Developmental Disorders Donor 1104
tissue
adipose
Sex
male
age
adult
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
We homogenized 20 mg of frozen pulverized adipose tissue in nuclei isolation buffer (20 nM Tris-HCl, 50 mM EDTA, 5mM spermidine, 0.15 mM spermine, 0.1% beta meracptoethanol, 40% glycerol, 1% NP40, pH 7.5) with a dounce homogenizer. The homogenate was centrifuged at 1,100 g for 10 minutes at 4C and the pellets resuspended in resuspension buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, pH 7.4). We ran tagmentation reactions at 37C for 30 minutes, purified samples with Qiagen MinElute kits, and amplified libraries with NEB NextPCR