GSM3494218: DLS395 CEH-60 3F10 IP r2; Caenorhabditis elegans; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Adult
Cell type
Adult
NA
NA
Attributes by original data submitter
Sample
source_name
Whole animals
strain
DLS395
developmental stage
Day 1 adults
genotype
ceh-60(rhd116)
chip antibody
anti-HA (3F10, Sigma-Aldrich, 11867423001)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Animals were harvested in M9 buffer, washed, and ground to a find powder with a mortar and pestle in liquid Nitrogen. Whole animal lysates were crosslinked with 1% formaldehyde for 5 minutes, and the genomic DNA was sheared using a Covaris E220 (Covaris). The resulting chromatin was immunoprecipitated with anti-HA or anti-FLAG antibodies. ChIP-Seq libraries were prepared for sequencing using the ThruPLEX DNA-Seq Kit (Rubicon Genomics) according to the manufacturer's instructions.